Used extensively as a fluorescent labeling reagent in biochemical and molecular biology research. It is particularly effective for labeling proteins, peptides, and other biomolecules due to its high reactivity with primary amines. The compound’s fluorescence properties make it suitable for applications such as fluorescence microscopy, flow cytometry, and immunoassays. It is also employed in tracking and imaging studies to monitor cellular processes and interactions. The succinimidyl ester group ensures efficient and stable conjugation with target molecules, enhancing its utility in sensitive detection methods.