DAF-2DA is widely used in biological research as a fluorescent indicator for the detection of nitric oxide (NO) in living cells and tissues. Its application is particularly valuable in studying the role of nitric oxide in various physiological and pathological processes, such as vascular function, neurotransmission, and immune response. The compound penetrates cell membranes easily and, upon interaction with NO, produces a highly fluorescent product, allowing researchers to visualize and quantify NO levels in real-time. This makes DAF-2DA a critical tool in understanding cellular signaling pathways and the impact of NO in diseases like hypertension, atherosclerosis, and neurodegenerative disorders. Additionally, it is employed in pharmacological studies to evaluate the efficacy of NO-modulating drugs.