Calcein Blue is widely used as a fluorescent dye in biological and chemical research. It is particularly valued for its ability to bind with metal ions, making it a useful tool for detecting and quantifying calcium and other divalent cations in various samples. In cell biology, it is employed to study calcium dynamics within living cells, providing insights into cellular processes such as signaling and apoptosis. Additionally, Calcein Blue is utilized in fluorescence microscopy and flow cytometry for visualizing and analyzing cell populations. Its stability and bright fluorescence also make it suitable for use in assays and diagnostic applications, where it helps in monitoring enzymatic activities and detecting specific biomarkers.