Widely used in biochemical research for labeling and detecting amines and sulfhydryl groups due to its strong fluorescence. Commonly employed in the analysis of amino acids, peptides, and proteins through fluorescence tagging, enabling sensitive detection in HPLC and capillary electrophoresis. Also used to study protein conformation and dynamics, as its fluorescence properties change in response to environmental polarity. Frequently applied in the determination of primary amines in pharmaceuticals and biological samples. Its high sensitivity makes it suitable for trace-level analysis in forensic and environmental applications.