Widely used as a pre-column derivatization reagent in HPLC analysis for the sensitive detection of primary and secondary amines, especially in biological samples. It reacts rapidly with amino groups under mild conditions to form highly fluorescent derivatives, enabling low-level quantification of amino acids, neurotransmitters, and aminergic drugs. The resulting labeled compounds exhibit excellent stability and strong fluorescence, making them ideal for fluorescence detection in reversed-phase HPLC systems. Its high selectivity for amines and compatibility with aqueous buffers enhance its utility in automated and high-throughput analytical workflows.