The chemical is widely used as a fluorescent derivatization reagent for the analysis of chiral amines and amino acids in high-performance liquid chromatography (HPLC). It reacts selectively with primary and secondary amines to form stable thiourea derivatives, which can be detected with high sensitivity due to their strong fluorescence. This property makes it particularly useful in the field of pharmaceutical analysis, where it helps in the quantification and enantiomeric separation of chiral drugs. Additionally, it is applied in biological research for labeling and detecting amino compounds in complex matrices, such as biological fluids and tissue samples. Its chiral selectivity ensures accurate identification and measurement of enantiomers, which is crucial for understanding their biological activity and pharmacokinetics.