Used primarily as a fluorescent dye in biological research, this chemical is applied in membrane potential studies. It helps in monitoring changes in membrane potential across cell membranes by exhibiting fluorescence changes in response to voltage shifts. This makes it valuable in neuroscience and physiology for studying the electrical properties of cells. Additionally, it is utilized in flow cytometry and fluorescence microscopy to assess cellular health and activity, providing insights into cell viability and function. Its sensitivity to membrane potential alterations makes it a crucial tool in understanding cellular dynamics and responses to various stimuli.