Coumarin 525 is widely used as a fluorescent dye in various applications due to its strong emission in the green spectrum. It is commonly employed in laser dyes, where it serves as an active medium in dye lasers, enabling the generation of tunable laser light. Additionally, it is utilized in biochemistry and molecular biology as a fluorescent marker for tracking and imaging biological molecules. In the field of material science, it is incorporated into organic light-emitting diodes (OLEDs) and other optoelectronic devices to enhance their performance. Its photostability and bright fluorescence also make it suitable for use in analytical chemistry for detecting and quantifying substances in complex mixtures.