Atto 425 is a fluorescent dye widely used in bioimaging and molecular labeling due to its high fluorescence quantum yield and photostability. It is commonly applied in fluorescence microscopy, enabling clear visualization of cellular structures and biomolecules. Its excitation and emission profile make it suitable for use with standard UV-blue light sources and detection systems, allowing seamless integration into multicolor imaging setups.

The dye is often conjugated to nucleotides, proteins, or antibodies for use in techniques such as DNA sequencing, immunoassays, and fluorescence in situ hybridization (FISH). Its good water solubility and minimal fluorescence quenching in aqueous environments enhance its performance in biological assays. Additionally, Atto 425 is employed in flow cytometry and single-molecule detection due to its bright and stable signal, supporting sensitive and precise analysis in research and diagnostic applications.