Nuclear yellow is widely used in biological research as a fluorescent tracer for studying cell dynamics and neuronal networks. It is particularly valuable in neuroscience for mapping neural pathways and visualizing intracellular structures. The dye’s ability to permeate cell membranes makes it useful for live-cell imaging, allowing researchers to track cell movement and interactions in real time. Additionally, it is employed in conjunction with other fluorescent markers to provide detailed insights into cellular processes and tissue organization. Its stability and bright fluorescence make it a reliable tool for advanced microscopy techniques.