This chemical is widely used in biochemical and biomedical research due to its unique properties. It is primarily employed as a fluorescent probe for imaging and tracking biological molecules in live cells and tissues. The benzothiazole Cy5 component provides strong fluorescence in the red to near-infrared range, making it ideal for deep tissue imaging with minimal background interference.

The PEG (polyethylene glycol) chains enhance the solubility and biocompatibility of the compound, allowing it to be used in various biological environments without causing significant toxicity or aggregation. The m-PEG4 and Acid-PEG3 groups also facilitate conjugation with target molecules, such as proteins, peptides, or antibodies, enabling specific labeling and detection in complex biological systems.

Applications include studying cellular processes, monitoring drug delivery, and visualizing molecular interactions in real-time. Its stability and fluorescence efficiency make it a valuable tool in fluorescence microscopy, flow cytometry, and in vivo imaging studies. Additionally, it is used in the development of diagnostic assays and therapeutic agents, where precise molecular tracking is essential.