Used in biochemical research, particularly in the study of molecular interactions and cellular processes. It serves as a fluorescent probe due to the pyrene moiety, enabling the visualization and tracking of molecules in various biological systems. The PEG5 spacer enhances solubility and reduces steric hindrance, making it suitable for conjugation with proteins, peptides, or other biomolecules. Applied in drug delivery systems to monitor the distribution and release of therapeutic agents. Also utilized in material science for developing fluorescently labeled polymers or surfaces. Its properties make it valuable in assays, imaging techniques, and nanotechnology applications.