This chemical is widely used in bioconjugation and labeling applications, particularly in the field of molecular biology and biochemistry. It serves as a reactive dye for attaching fluorescent markers to proteins, peptides, and other biomolecules, enabling visualization and tracking in various assays. Its NHS ester group allows for efficient covalent bonding with primary amines, making it ideal for labeling antibodies, enzymes, and other amine-containing molecules. The PEG5 spacer enhances solubility and reduces steric hindrance, improving the efficiency of labeling reactions. It is commonly employed in fluorescence microscopy, flow cytometry, and immunofluorescence studies to study cellular processes, protein interactions, and localization. Additionally, it is used in diagnostic applications, such as the development of fluorescent probes for detecting specific biomarkers in biological samples.