This chemical is widely used in bioconjugation and labeling applications due to its dual functional groups. The azide group allows for click chemistry reactions, particularly copper-catalyzed azide-alkyne cycloaddition (CuAAC), enabling the attachment of molecules to surfaces or other biomolecules. The fluorescein component provides strong fluorescence, making it suitable for fluorescence microscopy, flow cytometry, and other imaging techniques to track and visualize biological processes. The PEG (polyethylene glycol) spacers enhance solubility and reduce steric hindrance, improving the efficiency of conjugation. It is commonly employed in the study of protein-protein interactions, cellular labeling, and the development of targeted drug delivery systems. Additionally, its water solubility and biocompatibility make it ideal for use in biological and pharmaceutical research.