Used as a fluorescent probe in biochemical and analytical applications due to its strong fluorescence properties. It binds selectively to specific proteins or enzymes, making it useful in studying enzyme activity and molecular interactions. Commonly employed in assays for monitoring esterase activity, where enzymatic cleavage releases the fluorescent chromone derivative, allowing real-time detection. Also applied in cellular imaging for tracking biological processes in live cells. Its thiophene and pyrrole moieties enhance stability and binding specificity, improving sensitivity in detection systems.