H-Tyr(3-F)-OH is primarily utilized in peptide synthesis and pharmaceutical research. It serves as a modified amino acid building block, enabling the incorporation of fluorine into peptides, which can enhance their stability, bioavailability, and interaction with biological targets. This compound is particularly valuable in the development of peptide-based drugs, where fluorination can improve metabolic resistance and binding affinity. Additionally, it is used in biochemical studies to investigate the role of tyrosine residues in protein function and signaling pathways. Its application extends to the design of fluorescent probes and imaging agents for studying cellular processes.