4,4'-Difluorochalcone is primarily used in organic synthesis and pharmaceutical research. It serves as a key intermediate in the development of various bioactive compounds, particularly those targeting anti-inflammatory and anticancer properties. Its chalcone structure is often modified to explore structure-activity relationships in drug discovery. Additionally, it is utilized in materials science for the synthesis of organic electronic materials due to its conjugated system, which can enhance optoelectronic properties. Researchers also employ it in photochemical studies to understand light-induced reactions and energy transfer processes.