Sudan II is primarily used as a dye in various industrial applications. It is commonly employed in the coloring of solvents, oils, waxes, and plastics, providing a vibrant red-orange hue. In the petroleum industry, it is utilized to differentiate between different types of fuels and lubricants. Additionally, Sudan II finds application in biological staining, particularly in histology, to identify lipids and fats in tissue samples. However, due to its potential health risks, its use in food products is strictly prohibited. It is also employed in research laboratories as a chemical marker or tracer in various experiments.