Used in environmental and pharmaceutical research to detect sulfonamide antibiotics in water, soil, and biological samples. The ELISA kit format allows for high sensitivity and specificity, enabling rapid screening of sulfonamide residues in food products like milk, honey, and meat to ensure compliance with safety regulations. Also employed in monitoring wastewater treatment efficiency and studying antibiotic persistence in ecosystems. Suitable for lab-based analysis due to its ease of use, low sample volume requirement, and ability to process multiple samples simultaneously.