This compound is widely used in bioconjugation processes, particularly for attaching polyethylene glycol (PEG) chains to proteins, peptides, or other biomolecules. Its NHS ester group reacts efficiently with primary amines, enabling stable amide bond formation, while the PEG spacer enhances solubility and reduces immunogenicity of the conjugated molecules. It is commonly applied in drug delivery systems to improve the pharmacokinetics and stability of therapeutic agents. Additionally, it is utilized in the development of diagnostic probes and imaging agents, where PEGylation helps prolong circulation time and enhance targeting efficiency. The compound is also valuable in surface modification of nanoparticles and biomaterials to improve biocompatibility and reduce nonspecific interactions.