This compound is widely used in bioconjugation and crosslinking applications due to its reactive NHS ester groups, which efficiently bind to primary amines in proteins, peptides, and other biomolecules. It is particularly valuable in creating stable amide bonds, enabling the modification of biomolecules for research, diagnostics, and therapeutic purposes. Its PEG spacer enhances solubility, reduces immunogenicity, and improves biocompatibility, making it suitable for drug delivery systems, antibody-drug conjugates, and surface functionalization of nanoparticles. Additionally, it is employed in the development of biosensors and affinity chromatography matrices, where precise biomolecule attachment is critical.