This compound is widely used in bioconjugation and protein modification due to its reactive ester group, which facilitates efficient coupling with amine-containing molecules. It is particularly valuable in the development of antibody-drug conjugates (ADCs) and targeted drug delivery systems, where it helps link therapeutic agents to antibodies or other targeting molecules. The maleimide group allows for selective conjugation with thiol groups, making it ideal for attaching molecules to cysteine residues in proteins or peptides. Its PEG spacer enhances solubility and reduces steric hindrance, improving the stability and functionality of the resulting conjugates. Additionally, it is employed in the preparation of fluorescent probes, biosensors, and other biotechnological tools for research and diagnostic applications.