This chemical is widely used in bioconjugation and biochemical research due to its dual functional groups. The amino group allows for coupling with carboxyl groups or other reactive sites, while the biotin moiety enables strong binding to avidin or streptavidin, facilitating detection or purification processes. Its PEG4 spacers enhance solubility and reduce steric hindrance, making it ideal for labeling proteins, peptides, or other biomolecules. It is particularly useful in immunoassays, affinity chromatography, and the development of biotinylated probes for diagnostic or therapeutic applications. Additionally, its structure supports the creation of stable bioconjugates for targeted drug delivery systems.