This chemical is widely used in bioconjugation and labeling applications due to its unique properties. The DBCO (dibenzocyclooctyne) group enables click chemistry reactions with azide-containing molecules, allowing for efficient and specific conjugation without the need for catalysts. The biotin moiety facilitates strong binding to streptavidin or avidin, making it valuable in affinity purification, detection, and immobilization processes. The PEG4 spacers enhance solubility and reduce steric hindrance, improving the efficiency of binding interactions. The NHS ester group reacts with primary amines, enabling covalent attachment to proteins, peptides, or other amine-containing biomolecules. This makes it a versatile tool in applications such as protein labeling, drug delivery, and the development of diagnostic assays. Its multifunctional design is particularly useful in creating targeted bioconjugates for research and therapeutic purposes.