This compound is primarily utilized in biochemical research, particularly in the field of protein labeling and conjugation. It serves as a versatile linker or cross-linking agent, enabling the attachment of specific molecules, such as fluorescent dyes or affinity tags, to proteins or peptides. Its structure, featuring a reactive N-hydroxysuccinimide (NHS) ester group and a dinitrophenyl (DNP) moiety, allows for efficient and selective binding to amino groups in biomolecules. This makes it valuable in applications like immunoassays, where precise labeling is essential for detection and analysis. Additionally, its polyethylene glycol (PEG) spacer enhances solubility and reduces steric hindrance, improving the efficiency of conjugation processes.