This compound is primarily used in the field of bioconjugation and chemical biology, where it serves as a linker or spacer molecule. Its structure, featuring a polyethylene glycol (PEG) chain, enhances solubility and reduces immunogenicity, making it valuable in the development of drug delivery systems. It is often employed to conjugate therapeutic agents, such as antibodies or small molecules, to improve their pharmacokinetic properties and target specificity. Additionally, it finds application in the synthesis of peptide-based drugs, where the t-butyl ester group provides protection for carboxylic acid functionalities during chemical reactions. Its versatility also extends to the preparation of bioconjugates for diagnostic imaging and research tools in molecular biology.