m-PEG16-SH is widely used in bioconjugation and surface modification due to its thiol-reactive group and polyethylene glycol (PEG) chain. It is commonly applied in the modification of proteins, peptides, and nanoparticles to improve their solubility, stability, and biocompatibility. The thiol group allows for specific attachment to cysteine residues or gold surfaces, making it valuable in creating targeted drug delivery systems and biosensors. Additionally, it is used in the development of hydrogels and other biomaterials for tissue engineering, where its PEG segment helps reduce immune response and prevent protein adsorption. Its applications also extend to research in nanotechnology and diagnostics, where it aids in enhancing the performance and functionality of various biomedical tools.