NH-bis(peg4-t-butyl ester) is widely used in the field of bioconjugation and drug delivery systems. Its PEG (polyethylene glycol) chains enhance solubility and stability, making it valuable for modifying proteins, peptides, and other biomolecules. This compound is particularly useful in creating linkers for antibody-drug conjugates (ADCs), where it helps improve the pharmacokinetics and reduce immunogenicity of therapeutic agents. Additionally, its t-butyl ester groups provide protection during synthetic processes, allowing for controlled release or activation of functional groups in targeted drug delivery applications. It is also employed in the development of hydrogels and biomaterials for tissue engineering due to its biocompatibility and ability to form cross-linked networks.