S-Acetyl-PEG6-t-butyl ester is widely used in the field of bioconjugation and drug delivery. Its primary application lies in its ability to serve as a linker molecule, facilitating the attachment of various therapeutic agents, such as drugs or proteins, to other molecules or surfaces. The acetyl group provides stability during synthesis, while the PEG (polyethylene glycol) spacer enhances solubility and reduces immunogenicity, making it ideal for improving the pharmacokinetic properties of drugs. The t-butyl ester group can be selectively removed under mild conditions, allowing for controlled release or further functionalization. This compound is particularly valuable in the development of antibody-drug conjugates (ADCs), where it helps create stable and effective linkages between antibodies and cytotoxic agents. Additionally, it is utilized in the modification of peptides and proteins to enhance their stability and bioavailability in therapeutic applications.