This compound is widely used in bioconjugation and labeling applications due to its biotin and PEG3 components. The biotin moiety allows for strong binding to avidin or streptavidin, making it valuable in affinity-based assays, such as ELISA, pull-down assays, and protein purification. The PEG3 spacer enhances solubility and reduces steric hindrance, improving accessibility during binding interactions. The hydrazide group enables specific conjugation to aldehydes or ketones, facilitating the labeling of glycoproteins, carbohydrates, or other biomolecules with aldehyde groups. It is particularly useful in the preparation of biotinylated probes for diagnostic, imaging, and research purposes.