This compound is widely used in bioconjugation processes, particularly for linking biomolecules such as proteins, peptides, or antibodies to other entities like dyes, drugs, or solid supports. Its NHS ester group reacts efficiently with primary amines, forming stable amide bonds, while the maleimide group allows for selective thiol conjugation. This dual functionality makes it valuable in creating targeted drug delivery systems, diagnostic probes, and functionalized materials for research and therapeutic applications. It is especially useful in antibody-drug conjugate (ADC) development, where precise and stable linkages are critical. Additionally, it plays a role in surface modification of nanoparticles and polymers for biomedical applications.