This chemical is primarily used in bioconjugation and labeling applications in biochemistry and molecular biology. It serves as a versatile linker due to its azide group, which allows for click chemistry reactions, particularly with alkyne-containing molecules. The PEG3 spacer enhances solubility and reduces steric hindrance, making it suitable for attaching to biomolecules without disrupting their function. The desthiobiotin moiety enables reversible binding to streptavidin or avidin, facilitating purification or detection processes. It is commonly employed in protein labeling, pull-down assays, and affinity chromatography to study protein interactions, post-translational modifications, or cellular localization. Its design ensures efficient and specific conjugation, making it a valuable tool in proteomics and drug discovery research.