The compound is primarily used in bioconjugation and chemical biology research. It serves as a linker or spacer molecule, enabling the attachment of biomolecules such as peptides, proteins, or antibodies to other functional groups or surfaces. Its PEG (polyethylene glycol) chain enhances solubility and reduces immunogenicity, making it valuable in drug delivery systems and therapeutic applications. The t-butyl ester group provides stability during synthesis and can be selectively deprotected to yield a free carboxylic acid, allowing further functionalization. This makes it a versatile tool in the development of targeted therapies, diagnostic agents, and biomaterials.