This chemical is widely used in click chemistry applications, particularly in bioconjugation and labeling processes. Its TCO (trans-cyclooctene) group reacts efficiently with tetrazines via inverse electron-demand Diels-Alder reactions, enabling rapid and selective binding. The PEG4 (polyethylene glycol) spacer enhances solubility and reduces steric hindrance, making it suitable for use in biological systems. The DBCO (dibenzocyclooctyne) group allows for additional conjugation with azide-containing molecules through strain-promoted alkyne-azide cycloaddition (SPAAC). This dual functionality makes it valuable in fields like drug delivery, where it can be used to attach therapeutic agents to targeting molecules, and in imaging, where it helps in labeling biomolecules for tracking and visualization. Its versatility also extends to material science, where it aids in surface functionalization and the development of biomaterials.