This chemical is widely used in bioconjugation and click chemistry applications. It is particularly valuable for linking biomolecules, such as proteins, peptides, or antibodies, to other molecules or surfaces. The NHS ester group enables efficient coupling with primary amines, while the DBCO (dibenzocyclooctyne) group allows for rapid and selective reactions with azide-containing compounds via strain-promoted alkyne-azide cycloaddition (SPAAC). The PEG12 spacer provides flexibility and solubility, reducing steric hindrance and improving the efficiency of the conjugation process. It is commonly employed in drug delivery systems, diagnostic assays, and the development of bioconjugate therapeutics.