This compound is widely used in bioconjugation and click chemistry applications due to its dual functionality. The DBCO (dibenzocyclooctyne) group enables strain-promoted azide-alkyne cycloaddition (SPAAC), a fast and selective reaction with azides, making it ideal for labeling biomolecules without the need for catalysts. The NHS ester group reacts efficiently with primary amines, allowing for covalent attachment to proteins, peptides, or other amine-containing molecules. Its PEG5 spacer enhances solubility and reduces steric hindrance, improving reactivity in biological systems. It is commonly employed in drug delivery systems, antibody-drug conjugates, and surface modification of nanoparticles for targeted therapies. Additionally, it is used in biophysical studies, such as fluorescence labeling and imaging, to track molecular interactions in live cells or tissues. Its versatility makes it a valuable tool in chemical biology and materials science research.