This heterobifunctional linker, Mal-amido-PEG10-C2-NHS ester, is widely used in bioconjugation processes for attaching proteins, peptides, or other biomolecules to various surfaces or molecules. The maleimide group enables selective reaction with sulfhydryl (-SH) groups on cysteines, while the NHS ester reacts efficiently with primary amines, allowing site-specific covalent bonding to biomolecules like antibodies or enzymes. Its PEG10 (polyethylene glycol) spacer provides flexibility, hydrophilicity, and solubility, reducing steric hindrance, immunogenicity, and improving the stability and circulation time of the conjugated product.

This makes it valuable in drug delivery systems, where it helps create targeted therapies by linking drugs to specific carriers via thiol-amine conjugation. It is also utilized in diagnostic assays and imaging applications, aiding in the immobilization or labeling of biomolecules onto solid supports for accurate detection. Additionally, its application extends to the development of bioconjugates for research purposes, facilitating the study of molecular interactions and cellular processes.