This chemical is widely used in bioconjugation processes, particularly for labeling proteins, antibodies, and other biomolecules. Its PEG spacer enhances solubility and reduces steric hindrance, making it ideal for attaching biotin to target molecules in aqueous environments. The NHS ester group reacts efficiently with primary amines, enabling stable amide bond formation. This compound is commonly applied in immunoassays, such as ELISA, and in affinity chromatography for streptavidin-biotin interactions. It is also utilized in cell surface labeling and drug delivery systems, where biotinylation is required for targeting or detection purposes. Its versatility makes it a valuable tool in molecular biology and biochemical research.