Used in bioconjugation and labeling applications, particularly in the development of biotinylated probes and reagents for biochemical and biomedical research. It serves as a linker molecule, enabling the attachment of biotin to various biomolecules, such as proteins, peptides, or nucleic acids, for detection, purification, or immobilization purposes. The PEG6 spacer enhances solubility and reduces steric hindrance, improving binding efficiency in assays like ELISA, Western blotting, or affinity chromatography. It is also employed in drug delivery systems to create targeted therapies by conjugating biotin with therapeutic agents, leveraging the strong biotin-streptavidin interaction for precise targeting.