This chemical is widely used in bioconjugation techniques, particularly for labeling proteins, peptides, and other biomolecules with biotin. Its primary application lies in creating biotinylated compounds, which are essential for various biochemical assays and diagnostic tools. The biotin tag allows for strong and specific binding to avidin or streptavidin, enabling detection, purification, or immobilization of the labeled molecules. It is commonly employed in techniques such as ELISA, Western blotting, and immunohistochemistry, where biotinylation enhances sensitivity and specificity. Additionally, it is used in affinity chromatography to isolate and purify target proteins or nucleic acids. Its water-soluble nature and stable amide bond formation make it a reliable reagent for modifying biomolecules without compromising their functionality. This compound is particularly valuable in research and diagnostics for studying protein interactions, cellular processes, and disease markers.