NHS-PEG-NHS is widely used in bioconjugation and crosslinking applications due to its reactive NHS ester groups. It is commonly employed to link proteins, peptides, or other amine-containing molecules to surfaces or other biomolecules, facilitating the creation of stable amide bonds. This compound is particularly useful in the development of drug delivery systems, where it helps conjugate therapeutic agents to carriers like nanoparticles or polymers. It is also utilized in surface modification for biosensors and bioassays, enhancing the immobilization of biomolecules for improved detection and analysis. Additionally, NHS-PEG-NHS plays a role in tissue engineering by enabling the functionalization of scaffolds with bioactive molecules to promote cell adhesion and growth. Its water-soluble PEG spacer ensures biocompatibility and reduces immunogenicity in biomedical applications.