ZLN024 is a potent, direct activator of AMP-activated protein kinase (AMPK), binding to the β1 subunit and promoting its phosphorylation independent of upstream kinases. Primarily used in research settings, it helps investigate AMPK's critical role in cellular energy homeostasis, glucose and lipid metabolism, autophagy, and mitochondrial function. It is valuable in studies of metabolic disorders such as type 2 diabetes and obesity, cardiovascular diseases, cancer, and neurodegenerative conditions like Alzheimer's disease. ZLN024 also aids in exploring AMPK as a therapeutic target for inflammation, ischemia-reperfusion injury, and aging-related pathologies.