Used extensively in biochemical research to study G-protein coupled receptors (GPCRs) and their signaling pathways. It acts as a non-hydrolyzable analog of GDP, effectively locking G-proteins in their inactive state, which helps in understanding the mechanisms of signal transduction. This compound is particularly valuable in experiments involving the activation and deactivation cycles of G-proteins, providing insights into cellular responses and receptor interactions. Additionally, it is employed in assays to investigate the effects of various ligands on GPCR activity, aiding in the development of therapeutic drugs targeting these receptors.