Used in neuroscience research to study neurosteroid modulation of GABA_A receptors, particularly in relation to anxiety, depression, and seizure disorders. It acts as a negative allosteric modulator, helping scientists understand inhibitory neurotransmission and the role of endogenous neurosteroids in brain function. Also employed in electrophysiological studies to probe receptor kinetics and channel gating. Its charged nature allows for controlled experimental conditions due to limited membrane permeability, enabling precise investigation of extracellular receptor effects.