WAY-300716 is primarily used in neuroscience research as a potent and selective positive allosteric modulator of the GABAA receptor, specifically targeting the α5 subunit. Its main application lies in studying cognitive functions, particularly memory and learning processes. Due to its selectivity, it has been utilized in preclinical models to investigate the role of α5-containing GABA receptors in conditions such as schizophrenia, Alzheimer’s disease, and age-related cognitive decline. Researchers use WAY-300716 to enhance synaptic plasticity and improve performance in cognitive behavioral tasks in animal models, making it a valuable tool compound for validating the therapeutic potential of modulating specific GABA receptor subtypes. It is not used clinically and remains restricted to experimental settings.