NB Medium is widely used in microbiology and molecular biology for the cultivation of bacteria, particularly Escherichia coli, in laboratory settings. It supports high-density bacterial growth, making it ideal for plasmid DNA production and recombinant protein expression. Due to its rich nutrient composition, it enables rapid cell proliferation, which is essential in cloning, gene expression studies, and biotechnology applications requiring large-scale bacterial cultures. It is also commonly used in antibiotic selection experiments and bacterial transformation protocols.