Used primarily in biochemical research, this compound serves as a substrate for enzymes such as β-D-ribofuranosidase and other glycosidases that hydrolyze β-ribofuranosidic bonds. It helps in studying enzyme activity and kinetics due to its fluorescent properties upon enzymatic cleavage, releasing the fluorescent 4-methylumbelliferone moiety. Additionally, it is employed in diagnostic assays to detect and measure enzyme levels in various biological samples, aiding in the identification of enzyme-related disorders, particularly those involving carbohydrate metabolism. Its application extends to high-throughput screening in drug discovery, where it assists in identifying potential enzyme inhibitors.