Chondroitin ABC Lyase is widely used in biochemical research and pharmaceutical applications. It is primarily employed to degrade chondroitin sulfate, dermatan sulfate, and hyaluronan into smaller oligosaccharides, aiding in the study of glycosaminoglycan (GAG) structures and their biological functions. This enzyme is crucial in analyzing GAG composition in tissues, which helps in understanding their role in cell signaling, tissue development, and disease progression. Additionally, it is utilized in the production of low-molecular-weight heparins and other GAG-based therapeutics. In the medical field, Chondroitin ABC Lyase is used to investigate cartilage-related disorders, such as osteoarthritis, by breaking down cartilage components for analysis. Its ability to modify GAGs also makes it valuable in the development of biomaterials for tissue engineering and drug delivery systems.