L-threo-PPMP is primarily used in biochemical research to study glycosphingolipid metabolism. It acts as an inhibitor of glucosylceramide synthase, an enzyme crucial for the synthesis of glycosphingolipids. By inhibiting this enzyme, L-threo-PPMP helps researchers understand the role of glycosphingolipids in cellular processes and their involvement in various diseases, such as Gaucher's disease and other lysosomal storage disorders. Additionally, it is utilized in studies exploring potential therapeutic strategies for these conditions by targeting glycosphingolipid production. Its application extends to investigating lipid raft formation and function in cell membranes, providing insights into cell signaling and membrane dynamics.