GSK-25 is primarily utilized in the field of biomedical and pharmacological research, particularly in the study of cellular signaling pathways and mechanisms. As a tool compound and kinase inhibitor, it is employed to investigate the role of specific kinases in various biological processes, including cell proliferation, differentiation, and apoptosis. Researchers leverage GSK-25 to better understand disease mechanisms, especially in cancer, where dysregulated kinase activity promotes tumor growth and metastasis. Additionally, it is studied for potential applications in neurological disorders such as Alzheimer's and Parkinson's disease, due to its ability to modulate neuronal function and reduce neuroinflammation in the central nervous system. It is used in drug discovery efforts to identify and validate potential therapeutic targets, aiding in the development of novel kinase inhibitors. Its application extends to preclinical studies in laboratory and animal models, where it helps assess the efficacy and safety of new pharmacological interventions targeting kinase-related pathways before potential human use.